SILVER NANOPARTICLES AND RESISTANCE INDUCERS IN THE CONTROL OF BANANA ANTHRACNOSIS

Autores

  • Ana Paula Werkhausen Witter Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Joice Salla Carvalho Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Leandro Lunardi Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Bruno Pansera Espindola Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Marcos André Nohatto Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Patrícia Alcântara Gomes Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000
  • Fernanda Correa da Silva Vasconcellos Universidade Federal do Rio Grande do Sul Campus do Vale. Avenida Bento Gonçalves 9500 – Porto Alegre – RS - CEP 91501-970
  • Eliete de Fatima Ferreira da Rosa Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

DOI:

https://doi.org/10.28998/rca.v18i1.8062

Resumo

The search for techniques to control anthracnose (Colletotrichum musae) of lower toxicity to human health and environmental impact is essential for the development of a sustainable system in the banana production chain. Among these strategies, with great potential for use in agriculture and still lacking efficiency studies, silver nanoparticles (AgNPs) and resistance inducers stand out. Thus, the aim of this work was to evaluate the in vitro antifungal activity of AgNPs and resistance inducers in the control of post-harvest banana anthracnose. In in vitro experiment, the fungus was cultured in PDA culture medium added of different AgNP concentrations (0, 25, 50, 100 and 200 ppm) and resistance inducers (Bacillus subtilis, acibenzolar-S-methyl and Saccharomyces cerevisiae). The variable analyzed was mycelial growth inhibition (%), while in the post-harvest experiment, banana fruits were immersed for three minutes using the same treatments as the in vitro study. In this case, incidence (% of fruits with lesions) and severity (% of epidermis covered by lesions) were evaluated. Colloidal silver concentration at 100 ppm, as well as the resistance of Bacillus subtilis and acibenzolar-S-methyl inducers were the most efficient treatments in the control of post-harvest banana anthracnose caused by Colletotrichum musae.

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Biografia do Autor

Ana Paula Werkhausen Witter, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Discente do Curso de Engenharia Agronômica do IFC Campus Santa Rosa do Sul

Joice Salla Carvalho, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Discente do Curso de Engenharia Agronômica do IFC Campus Santa Rosa do Sul

Leandro Lunardi, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Engenheiro Químico. Técnico em laboratório do IFC Campus Santa Rosa do Sul

Bruno Pansera Espindola, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Eng. Agr. Doutor em Produção Vegetal. Professor do IFC Campus Santa Rosa do Sul

Marcos André Nohatto, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Eng. Agr. Doutor em Fitossanidade (Herbologia). Professor do IFC Campus Santa Rosa do Sul

Patrícia Alcântara Gomes, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Bióloga. Técnica em laboratório do IFC Campus Santa Rosa do Sul

Fernanda Correa da Silva Vasconcellos, Universidade Federal do Rio Grande do Sul Campus do Vale. Avenida Bento Gonçalves 9500 – Porto Alegre – RS - CEP 91501-970

Bióloga. Doutora em Engenharia de Bioprocessos e Biotecnologia da UFRGS Campus do Vale

Eliete de Fatima Ferreira da Rosa, Instituto Federal Catarinense – Campus Santa Rosa do Sul. Rua das Rosas s/n - Cx. Postal 04 – Santa Rosa do Sul – SC - CEP: 88965-000

Eng. Agr. Doutora em Manejo de Solos.  Professora do IFC Campus Santa Rosa do Sul

Referências

Agrios, G.N. Plant pathology. 5° ed. Holanda: Elsevier, Amsterdam; 2004.922p.

GEAGESP - Companhia de Entrepostos e Armazéns Gerais de São Paulo. 2015. Disponível em: <http://www.ceagesp.gov.br/wp-content/uploads/2015/07/banana.pdf>.Acesso em: 05 fev 2019.

Chen, L-H.; Han, R.; Zhang, H.; Xu, X-H.; Shao, H-B.; Wang, M-Y.; Cheng, Y.; Shang, X-H. Irrigating-continuous croppingwith Bacillus subtilis D9 fortified waste water could control the Fusarium wilt of Artemisia selengens. Applied Soil Ecology, 2017, 113, 127-134.

Conrath, U., Pieterse, C.M.J.; Mauch-Mani, B. Priming in plant pathogeninteractions. Trends in Plant Science. 2001, 7,5, 210-216.

FAOSTAT - Food and Agriculture Organization of the United Nations2017. Disponível em: . Acesso em: 05 fev 2019.

Ge, Y.; Deng, H.; Bi, Y.; Li, C.; Liu, Y.; Dong, B. Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit. Postharvest Biology and Technology, 2015, 99,1,160-167.

Ghini, R.; Bettiol, W. Proteção de plantas na agricultura sustentável. Cadernos de Ciência & Tecnologia, 2000, 17, 1, 61-67.

Gilardi, G.; Demarchi, S.; Gullino, M.L.; Garibaldi, A. Evaluation of the short term effect of nursery treatments with phosphite-based products, acibenzolar-S-methyl, pelleted Brassica carinata and biocontrol agents, against lettuce and cultivated rocket fusarium wilt under artificial inoculation and greenhouse conditions. Crop Protection, 2016, 85, 23-32.

Gond, S.K.; Bergen, M.S.; Torres, M.S.; White Jr., J.F. Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize. Microbiological Research, 2015, 172, 79-87.

Gupta, S.D.; Agarwal, A.; Pradhan, S. Phytostimulatory effect of silver nanoparticles (AgNPs) on rice seedling growth: An insight from antioxidative enzyme activities and gene expression patterns. Ecotoxicology and Environmental Safety, 2018, 161, 624-633.

Gurgel, L.M.S.; Coelho, R.S.B.; Oliveira, S.M.A.de.; Silva, R.L.X.da.; Rosa, R.C.T.da.; Assis, T.C.de.; Andrade, D.E.G.T.de. Efeito de indutores de resistência no controle da antracnose do bastão do imperador (Etlingera elatior). Anais da Academia Pernambucana de Ciência Agronômica, 2016, 13, 223-233.

IBGE - Instituto Brasileiro de Geografia e Estatística. Levantamento sistemático da produção agrícola, 2016. Disponível em:

<https://ww2.ibge.gov.br/home/estatistica/pesquisas/pesquisa_resultados.php?id_pesquisa=44>. Acesso em: 07 nov 2018.

Kholoud, M.M.; Abou, E.N.; Ala`a, E. Synthesis and applications of silver

nanoparticles. Arabian Journal of Chemistry, 2010, 3, 3, 35-140.

Kim, S.W.; Jung, J.H.; Lamsal, K.; Kim, Y.S.; Min, J.S.; Lee, Y.S.Antifungal effects of silver nanoparticles (AgNPs) against various plant pathogenic fungi. Mycobiology, 2018, 40, 1, 53-58.

Lamsal, K.; Kim, S.W.; Jung, J.H.; Kim, Y.S.; Kim, K.S.; Lee, Y.S. Application of silver nanoparticles for the control of Colletotrichum species in vitro and pepper anthracnose disease in field. Mycobiology, 2011, 39, 3, 194-199a.

Lamsal, K.; Kim, S.W.; Jung, J.H.; Kim, Y.S.; Kim, K.S.; Lee, Y.S. Inhibition effects of silver nanoparticles against powdery mildews on cucumber and pumpkin. Mycobiology. 2011, 39, 1, 26-32b.

Liu, Y.; Ge, Y.; Bi, Y.; Li, C.; Deng, H.; Hu, L.; Dong, B. Effect of postharvest acibenzolar-S-methyl dipping on phenylpropanoid pathway metabolism in muskmelon (Cucumis melo L.) fruits. Scientia Horticulturae, 2014, 168, 113-119.

Lopes, M.R.; Klein, M.N.; Ferraz, L.P.; Silva, A.C.da.; Kupper, K.C. Saccharomyces cerevisiae: A novel and efficient biological control agent for Colletotrichum acutatum during pre-harvest. Microbiological Research, 2015, 175, 93-99.

Maxson-Stein, K.; He, S-Y.; Hammerschmidt, R.; Jones, A.L. Effect of treating apple trees with acibenzolar-s-methyl on fire blight and expression of pathogenesis-related protein genes. Plant Disease, 2002, 86, 7, 785-790.

Min, J-S.; Kim, K-S.; Kim, S-W.; Jung, J-H.; Lamsal, K.; Kim, S-B.; Jung, M-Y.; Lee, Y-S. Effects of colloidal silver nanoparticles on Sclerotium-forming phytopathogenic fungi. The Plant Pathology Journal, 2009, 25, 4, 376-380.

Ouda, S.M. Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Research Journal of Microbiology, 2014, 9, 1, 34-42.

Prabhu, S.; Poulose, E.K. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications and toxicity effects. International Nano Letters, 2012, 32,1-10.

Pyoung II, K.; Ryu, J.; Kim, Y.H.; Chi, Y-T. Production of biosurfactant lipopeptides iturin A, fengycin, and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. Journal of Microbiology and Biotechnology, 2010, 20, 1, 138-145.

Silva, L.M.; Barbosa, M.G.; Fernandes, M.B.; Ribeiro, R.C.F.; Mizobutsi, E.H. Progresso temporal e controle da antracnose em banana no semiárido norte mineiro. Revista Brasileira de Fruticultura, 2016, 38, 1, 81-91.

Srikhong, P.; Lertmongkonthum, K.; Sowanpreecha, R.; Rerngsamran, P. Bacillus sp. strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici.BioControl, 2018, 63, 6, 833-842.

Stangarlin,J.R.;Kuhn, O.J.;Toledo,M.V.;Portz,R.L.; Schwan-Estrada, K.R.F.; Pascholati, S.F. A defesa vegetal contra fitopatógenos. Scientia Agraria Paranaenis, 2011, 10, 18-46.

Stella, F.P.; Steffens, C.A.; Amarante, C.V.T.; Martin, M.S. Maturação, amadurecimento de frutos e controle de podridões de Penicillium spp. em maçãs ‘Fuji’ com a aplicação pré-colheita de indutores de resistência. Revista de Ciências Agroveterinárias, 2013, 12, 1, 31-38.

Toffano, L.; Fialho, M.B.; Pascholati, S.F. Potential of fumigation of orange fruits with volatile organic compounds produced by Saccharomyces cerevisiae to control citrus black spot disease at postharvest. Biological Control, 2017, 8,1, 77-82.

Van Loon, L.C.; Van Strien,E.A. The families of pathogenesis-related proteins, their activities and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology, 1999, 55, 85-97.

Wang, X.; Wang, J.; Jin, P.; Zheng, Y. Investigating the efficacy of Bacillus subtilisM21 on controlling Rhizopus rot in peach fruit. International Journal of Food Microbiology, 2013,164, 141-147.

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Publicado

2020-04-12

Edição

Seção

Proteção de Plantas