IDENTIFICATION AND MICROPROPAGATION OF VIRUS-FREE BLACK PEPPER GENOTYPES (Piper nigrum L.)

Authors

  • Gleyce kelly Sousa Ramos Universidade Federal Rural da Amazônia - Ufra http://orcid.org/0000-0001-7344-1817
  • Oriel Filgueira de Lemos Embrapa Amazônia Oriental
  • Elisa Ferreira Moura Cunha Embrapa Amazônia Oriental
  • Alessandra de Jesus Boari Embrapa Amazônia Oriental
  • Danielle Pereira Mendonça Universidade Federal Rural da Amazônia
  • Lana Roberta Reis dos Santos Universidade Federal Rural da Amazônia
  • Simone de Miranda Rodrigues Embrapa Amazônia Oriental
  • Ilmarina Campos de Menezes Embrapa Amazônia Oriental

DOI:

https://doi.org/10.28998/rca.v18i1.7049

Abstract

Virus infections are a major constraint for black pepper cultivation (Piper nigrum L.), a vegetatively propagated crop; therefore, the in vitro selection of virus- free plants is an alternative to reestablish phytosanitary quality of pepper culture. The aim of this study was to select and micropropagate PYMoV virus-free pepper plants. ‘Apra’, ‘Bragantina’, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ genotypes were used in the indexing process. From these, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ were virus free, and only ‘Cingapura’ and ‘Clonada’ were selected for micropropagation. In the multiplication phase, ‘Cingapura’ genotype showed higher multiplication rate in relation to ‘Clonada’ in in vitro rooting and in acclimatization, response was similar in both genotypes. It was possible to identify virus-free genotypes of commercial interest, selecting them for micropropagation, and in this stage, influence of genotype is of great importance.

Downloads

Download data is not yet available.

References

Ahmad, N.; Guo, B.; Fazal, H.; Abbasi, B. H.; Liu, C. Z.; Mahmood, T., Shinwari, Z. K. Feasible plant regeneration in black pepper from petiole explants. Journal of Medicinal Plants Research. 2011, 5, 4590-4595.

Boari, A. de J. Evaluation of the active germplasm bank of consumption-kingdom for virosis and development of a control strategy. Embrapa Amazônia Oriental-Documentos (INFOTECA-E), 2008. (in portuguese)

Bhat, A. I.; Biju, C. N.; Srinivasan, V.; Krishnamurthy, S. A. K. Current status of viral diseases affecting black pepper and cardamom. Journal of Spices and Aromatic Crops, 2018, 27, 1-16.

Bhat, A. I.; Devasahayam; S.; Sarma; Y. R.; Pant, R. P. Association of a badnavirus in black pepper (Piper nigrum L.) transmitted by mealybug (Ferrisia virgata) in India. Current science, 2003, 1547-1550.

Bhat, A. I.; Siljo,A.; Jiby, M. V.; Thankamani, C. K.; Mathew, P. A. Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus. Journal of Spices and Aromatic Crops, 2009, 18, 28-32.

Carvalho, A. C. P. P.; Torres, A. C.; Braga, E. J. B.; De Lemos, E. E. P.; Souza, F. V. D.; Peters, J. A.; Câmara, T. R. Plant tissue culture glossary. Plant Cell Culture & Micropropagation, 2016, 7, 1, 30-60. (in portuguese)

Castro, G. L. S.; De Lemos, O. F.; Tremacoldi, C. R.; Moraes, F. K. C.; Dos Santos, L. R. R.; Pinheiro, H. A. Susceptibility of in vitro black pepper plant to the filtrate from a Fusarium solani f. sp. piperis culture. Plant Cell, Tissue and Organ Culture (PCTOC). 2016, 127, 263-268.

CONAB - Companhia Nacional de Abastecimento – Precos. Disponíveis em: http://sisdep.conab.gov.br/precosiagroweb/. 27/11/2018.

Duarte, M. De L.R.; Albuquerque, F.C. De; Poltronieri, L.S.; Trindade, D.R.; Kitajima, E.W.; Brioso, P.S.T. Yellow black pepper mottled. Embrapa Amazônia Oriental. Belém: Pará, 2000; Documentos, 62, 20p. (in portuguese)

Gibbs, A.; Mackenzie, A. A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of virology methods, 1997, 63, 9-16.

HAZARIKA, B. N. Morpho-physiological disorders in in vitro culture of plants. Scientia horticulturae, 2006, 108, 2, 105-120.

Huyen, D. A.; Du, T. X.; Giap, D. D.; Ton, N. T. Preliminary study on the micropropagation in vitro of black pepper (Piper nigrum L.). Tap Chi Sinh Hoc, 2014, 27, 39-45.

Instituto Brasileiro de Geografia e Estatística – IBGE. Sistema de recuperação automática – SIDRA. 2016. Disponível em: http://www.sidra.ibge.gov.br/bda/tabela/listabl.asp?c=1613&z=p&o=27. Acesso em: 26 de julho de 2019.

LEMOS, O. F. de. In vitro mutagenesis in the genetic improvement of black pepper (Piper nigrum L.). Tese de doutorado. Piracicaba - Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo. 2003. 191p. (in portuguese)

Lemos. O. F.; Poltronieri. M. C.; Rodrigues. S. De M.; Meneses. I. C. De; Mondin. M. Conservation and genetic improvement of black pepper (Piper nigrum L.) associated with biotechnology techniques. Embrapa Amazônia Oriental. Belém: Pará, 2011; Documentos, 375, 45p. (in portuguese)

Lemos, O. F.; Poltonieri, M. C. Seedling productio. In.: Lemos, O. F.; Tremacoldi, C. R.; Poltonieri, M. C (ed.). Good agricultural practices for increasing productivity and quality of black pepper in the state of Pará. Belém: Embrapa Amazônia Oriental, 2014. 52 p. (in portuguese)

Lourinho, M. P.; Costa, C. A. S Da; Souza, L. C. De; Souza, L. C. De; Oliveira Neto, C. F. de. Conjuncture of black pepper in the national market and in the northern region of Brazil. Enciclopédia Biosfera. Centro Científico Conhecer, 2014, 10, 1016-1031. (in portuguese)

Lockhart, B. E. L.; Kiratiya-Angul, K.; Jones, P.; Eng, L.; Silva, P.; Olszewski, N. E.; Lockhart, N.; Deema, N.; Sangalang, J. Identification of Piper yellow mottle virus, a mealybug-transmitted badnavirus infecting Piper spp. in Southeast Asia. European Journal of Plant Pathology, 1997, 103, 303-311.

Miftakhurohmah, N. F. N.; Mariana, Maya; Wahyuno, Dono. Deteksi Piper Yellow Mottle Virus (PYMOV) penyebab penyakit kerdil pada tanaman lada secara Polymerase Chain Reaction (PCR). Buletin Penelitian Tanaman Rempah dan Obat, 2016, 27, 77-84.

Moura, E. F.; Menezes, I. C.; Lemos, O. F. Micropropagation of black pepper (Piper nigrum). Plant Cell Culture & Micropropagation, 2009, 65-70.

Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 1962, 15, 473–497.

Philip, V. J.; Joseph, D.; Triggs; G. S.; Dickinson, N. M. Micropropagation of black pepper (Piper nigrum L.) through shoot tip cultures. Plant Cell Reports, 1992, 12, 42-44.

Sasi, Shina; Bhat, A. I. In vitro elimination of Piper yellow mottle virus from infected black pepper through somatic embryogenesis and meristem-tip culture. Crop Protection, 2018, 103, 39-45.

Serrano, L. A. L.; Marinato, F. A.; Magiero, M., Sturm, G. M. Production of black pepper seedlings in commercial substrate fertilized with slow release fertilizer. Revista Ceres, 2012, 59, 512-517. (in Portuguese)

Silva, D. P. P.; Jones, P.; Shaw, M. W. Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri Lanka. Plant Pathology, 2002, 51, 537–545.

Umadevi, P.; Bhat, A. I.; Krishnamurthy, K. S.; Anandaraj, M. Influence of temperature on symptom expression, detection of host factors in virus infected Piper nigrum L. Indian journal of experimental biology, 2016, 54, 5, 354-360.

Umadevi, P.; Saji, K. V.; Suraby, E. J. Meristem culture for rapid regeneration in Black pepper (Piper nigrum Linn.). Annals of Plant Sciences, 2015, 4, 1029-1032.

Published

2020-04-12

Issue

Section

Proteção de Plantas