VIABILITY CLASSES BY THE TETRAZOLIUM TEST OF PEANUT SEEDS CULTIVATED IN THE NORTHEAST

Authors

  • Késsia de Mendonça Santos Santos Universidade Federal de Alagoas
  • Elda Universidade Federal de Alagoas
  • Ludmila Lira da Silva
  • LUAN
  • João

DOI:

https://doi.org/10.28998/rca.22.18440

Keywords:

Arachis hypogaea L, Agricultural seeds., Tetrazolium test. , Force

Abstract

Peanut is one of the most economically important oilseeds, widely cultivated worldwide. However, its lipid-rich composition contributes to reduced seed performance. Therefore, this study aimed to propose a methodology for evaluating the viability of peanut seeds of the BR-1 variety by the tetrazolium test, using two seed preconditioning methods, immersion periods and adequate concentration of the tetrazolium solution, as well as determining specific viability classes for the BR-1 variety. The experimental design was completely randomized, in a 3x2 factorial scheme (three periods x two concentrations), and four replicates of 50 seeds for each combination of period and concentration, totaling 40 experimental plots per seed preconditioning method. The cotyledons containing the embryonic axis were placed in 50 mL plastic cups in the 2,3,5-triphenyl tetrazolium chloride salt solution for immersion periods of 1.5, 2.5, and 3.5 h, and at concentrations of 0.05 and 0.075%, in the B.O.D. incubator, in the dark at 30 °C. The viability of the seeds from each preconditioning method, filter paper and direct immersion, within each combination of period and concentration, was compared with the percentage of germinated seeds in the standard germination test. Thus, the concentration of 0.075% for 2.5 or 3.5 h, with 93 and 90% of viable seeds, respectively, did not differ significantly in relation to the germination percentage of the control, which was 91%. And classes 1, 2, 3 and 4 represented, respectively, the vigorous, viable, non-viable and dead seeds. It is worth mentioning that no studies indicating a methodology for evaluating the viability of peanut seeds adapted especially to the Northeast region were found. Preconditioning by direct immersion in water, manual separation of the cotyledons, and a concentration of 0.075% for 2.5 h in the tetrazolium test allow the evaluation of the viability of peanut seeds of the BR1 variety.

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References

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Published

2024-11-23

Issue

Section

VIII Semana da Agronomia - 1º Concurso científico